Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement
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Shafiee-2014-Nanostructured Optical.pdf
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1.07 MB
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Author(s)
Shafiee, Hadi
Lidstone, Erich A.
Jahangir, Muntasir
Inci, Fatih
Hanhauser, Emily
Henrich, Timothy J.
Kuritzkes, Daniel R.
Cunningham, Brian T.
Demirci, Utkan
Date Issued
February 2014
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Shafiee, Hadi, Erich A. Lidstone, Muntasir Jahangir, Fatih Inci, Emily Hanhauser, Timothy J. Henrich, Daniel R. Kuritzkes, Brian T. Cunningham, and Utkan Demirci. “Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement.” Sci. Rep. 4 (February 28, 2014).
Version
Final published version
Abstract
Detecting and quantifying biomarkers and viruses in biological samples have broad applications in early disease diagnosis and treatment monitoring. We have demonstrated a label-free optical sensing mechanism using nanostructured photonic crystals (PC) to capture and quantify intact viruses (HIV-1) from biologically relevant samples. The nanostructured surface of the PC biosensor resonantly reflects a narrow wavelength band during illumination with a broadband light source. Surface-adsorbed biotarget induces a shift in the resonant Peak Wavelength Value (PWV) that is detectable with <10 pm wavelength resolution, enabling detection of both biomolecular layers and small number of viruses that sparsely populate the transducer surface. We have successfully captured and detected HIV-1 in serum and phosphate buffered saline (PBS) samples with viral loads ranging from 10[superscript 4] to 10[superscript 8] copies/mL. The surface density of immobilized biomolecular layers used in the sensor functionalization process, including 3-mercaptopropyltrimethoxysilane (3-MPS), N-gamma-Maleimidobutyryl-oxysuccinimide ester (GMBS), NeutrAvidin, anti-gp120, and bovine serum albumin (BSA) were also quantified by the PC biosensor.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Creative Commons Attribution-Noncommercial
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DOI of Published Version
https://doi.org/10.1038/srep04116
