Compaction and segregation of sister chromatids via active loop extrusion
Name
Goloborodko-2016-Compaction and segre.pdf
Size
2.93 MB
Format
Adobe PDF
Checksum (MD5)
c6e89577aba4f118c0be458b68895a6a
Author(s)
Goloborodko, Anton
Imakaev, Maksim Viktorovich
Marko, John F.
Mirny, Leonid A.
Date Issued
May 2016
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Goloborodko, Anton, Maxim V Imakaev, John F Marko, and Leonid Mirny. "Compaction and segregation of sister chromatids via active loop extrusion." eLife 5 (2016):e14864.
Version
Final published version
Abstract
The mechanism by which chromatids and chromosomes are segregated during mitosis and meiosis is a major puzzle of biology and biophysics. Using polymer simulations of chromosome dynamics, we show that a single mechanism of loop extrusion by condensins can robustly compact, segregate and disentangle chromosomes, arriving at individualized chromatids with morphology observed in vivo. Our model resolves the paradox of topological simplification concomitant with chromosome 'condensation', and explains how enzymes a few nanometers in size are able to control chromosome geometry and topology at micron length scales. We suggest that loop extrusion is a universal mechanism of genome folding that mediates functional interactions during interphase and compacts chromosomes during mitosis.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.7554/eLife.14864
