Different Roles for Tet1 and Tet2 Proteins in Reprogramming-Mediated Erasure of Imprints Induced by EGC Fusion
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Piccolo_2013-Different roles for Tet1.pdf
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Author(s)
Piccolo, Francesco M.
Bagci, Hakan
Brown, Karen E.
Landeira, David
Soza-Ried, Jorge
Feytout, Amelie
Mooijman, Dylan
Hajkova, Petra
Leitch, Harry G.
Tada, Takashi
Date Issued
February 2013
Journal
Molecular Cell
Publisher
Elsevier
Citation
Piccolo, Francesco M., Hakan Bagci, Karen E. Brown, David Landeira, Jorge Soza-Ried, Amelie Feytout, Dylan Mooijman, et al. “Different Roles for Tet1 and Tet2 Proteins in Reprogramming-Mediated Erasure of Imprints Induced by EGC Fusion.” Molecular Cell 49, no. 6 (March 2013): 1023-1033. © 2013 Elsevier Inc.
Version
Final published version
Abstract
Genomic imprinting directs the allele-specific marking and expression of loci according to their parental origin. Differential DNA methylation at imprinted control regions (ICRs) is established in gametes and, although largely preserved through development, can be experimentally reset by fusing somatic cells with embryonic germ cell (EGC) lines. Here, we show that the Ten-Eleven Translocation proteins Tet1 and Tet2 participate in the efficient erasure of imprints in this model system. The fusion of B cells with EGCs initiates pluripotent reprogramming, in which rapid re-expression of Oct4 is accompanied by an accumulation of 5-hydroxymethylcytosine (5hmC) at several ICRs. Tet2 was required for the efficient reprogramming capacity of EGCs, whereas Tet1 was necessary to induce 5-methylcytosine oxidation specifically at ICRs. These data show that the Tet1 and Tet2 proteins have discrete roles in cell-fusion-mediated pluripotent reprogramming and imprint erasure in somatic cells.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is available under a Creative Commons license.
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2013.01.032
