Polymeric Nanoparticles Active against Dual-Species Bacterial Biofilms
Name
molecules-26-04958.pdf
Size
2.85 MB
Format
Unknown
Checksum (MD5)
0d394f8041dafd3bac349f2e8b5e541d
Author(s)
Makabenta, Jessa Marie V.
Park, Jungmi
Li, Cheng-Hsuan
Chattopadhyay, Aritra Nath
Nabawy, Ahmed
Landis, Ryan F.
Gupta, Akash
Schmidt-Malan, Suzannah
Patel, Robin
Rotello, Vincent M.
Date Issued
August 2021
Journal
Molecules
Publisher
Multidisciplinary Digital Publishing Institute
Citation
Molecules 26 (16): 4958 (2021)
Version
Final published version
Abstract
Biofilm infections are a global public health threat, necessitating new treatment strategies. Biofilm formation also contributes to the development and spread of multidrug-resistant (MDR) bacterial strains. Biofilm-associated chronic infections typically involve colonization by more than one bacterial species. The co-existence of multiple species of bacteria in biofilms exacerbates therapeutic challenges and can render traditional antibiotics ineffective. Polymeric nanoparticles offer alternative antimicrobial approaches to antibiotics, owing to their tunable physico-chemical properties. Here, we report the efficacy of poly(oxanorborneneimide) (PONI)-based antimicrobial polymeric nanoparticles (PNPs) against multi-species bacterial biofilms. PNPs showed good dual-species biofilm penetration profiles as confirmed by confocal laser scanning microscopy. Broad-spectrum antimicrobial activity was observed, with reduction in both bacterial viability and overall biofilm mass. Further, PNPs displayed minimal fibroblast toxicity and high antimicrobial activity in an in vitro co-culture model comprising fibroblast cells and dual-species biofilms of Escherichia coli and Pseudomonas aeruginosa. This study highlights a potential clinical application of the presented polymeric platform.
MIT Department
Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/molecules26164958
