BBF RFC #111: Inducible expression vector with His tag fusion for purification of RFC25 parts
Name
BBF_RFC_111.pdf
Size
153.7 KB
Format
Adobe PDF
Checksum (MD5)
8044bf4886fc0dcf170c4053811851b7
Author(s)
Husser, Mathieu
Narcross, Lauren
Sundarakrishnan, Adithi
Date Issued
August 24, 2017
Abstract
This Request For Comments (RFC) describes a new expression vector based on the pET expression system and designed for BioBrick RFC25 parts. This design includes all the features of pET expression vectors with a new origin of replication, resistance marker and Multiple Cloning Site (MCS). This MCS contains new restriction sites and a 5’ poly- histidine tag that allows the purification of expressed RFC25 parts. Previous attempts have been prevented by restriction site incompatibility, but using RFC25 parts and their restriction sites enables 5’ fusion to these parts. This new expression vector enables the cloning of RFC25 parts with a 5’ His tag in a single step. This allows the inducible expression of these parts and the easy purification of protein products.
Subjects
BioBricks Technical Standards Request for Comments
Terms of Use
Attribution-NoDerivs 3.0 United States
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