Illuminating the function of a master regulator of Caulobacter crescentus gene expression
Name
1008758218-MIT.pdf
Description
Full printable version
Size
2.63 MB
Format
Adobe PDF
Checksum (MD5)
9b985d48fa2e63124ec01414d5bca633
Author(s)
Yuan, Andy H. (Andy Han)
Advisor(s)
Michael T. Laub.
Date Issued
2012
Publisher
Massachusetts Institute of Technology
Abstract
Bacteria must allocate cellular resources required for transcription in a manner that accommodates the temporally regulated expression of functionally specialized gene products and the constitutive synthesis of stable RNAs required for ribosome biogenesis and translation. The Caulobacter crescentus cell cycle has been well-characterized and represents a powerful model system for studying the regulation of global gene expression patterns and "just-in-time" transcription. Here, we provide evidence that the Caulobacter GcrA protein directly regulates transcription at active promoters of highly expressed genes, particularly those encoding stable RNAs. Furthermore, we demonstrate that GcrA chromosome occupancy is dynamic and subject to epigenetic regulation. Our findings establish GcrA as an integral regulator of bacterial physiology and development.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2012.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 25-27).
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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