An isoperibol calorimeter for the investigation of biochemical kinetics and isothermal titration calorimetry
Name
191676863-MIT.pdf
Description
Full printable version
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2.57 MB
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Checksum (MD5)
5035f275035617e7d640f81ef05de9d9
Author(s)
Amadi, Ovid Charles
Advisor(s)
Ian W. Hunter.
Date Issued
2007
Publisher
Massachusetts Institute of Technology
Abstract
Isothermal titration calorimetry is a technique used to measure the enthalpy change associated with a molecular binding interaction. From these data, the binding constant for the reaction can be determined. In the scope of a larger project to design a high sensitivity instrument for collecting such data, the current methods in isothermal titration calorimetry were investigated. Further calorimetric experience was acquired by designing a large scale calorimetric device. Dilution reactions with dimethyl sulfoxide and water were conducted to measure the excess enthalpy of binding. The inaccuracy of these measurements necessitated the more careful design of an isoperibol calorimeter. This calorimeter was modeled was an arrangement of coupled thermal masses and capacitances in order to fully understand its transient response to a thermal input. Dilution reactions and a neutralization reaction with HCl and NH40H were performed on the system and the results were used to make recommendations for the design of the future high sensitivity device.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
Includes bibliographical references (leaf 52).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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