Control of PNG kinase, a key regulator of mRNA translation, is coupled to meiosis completion at egg activation
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elife-22219-v1.pdf
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2.25 MB
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Author(s)
Hara, Masatoshi
Petrova, Boryana
Orr-Weaver, Terry
Date Issued
May 2017
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Hara, Masatoshi et al. “Control of PNG Kinase, a Key Regulator of mRNA Translation, Is Coupled to Meiosis Completion at Egg Activation.” eLife 6 (May 2017): e22219. © Hara et al.
Version
Final published version
Abstract
The oocyte-to-embryo transition involves extensive changes in mRNA translation, regulated in Drosophila by the PNG kinase complex whose activity we show here to be under precise developmental control. Despite presence of the catalytic PNG subunit and the PLU and GNU activating subunits in the mature oocyte, GNU is phosphorylated at Cyclin B/CDK1sites and unable to bind PNG and PLU. In vitro phosphorylation of GNU by CyclinB/CDK1 blocks activation of PNG. Meiotic completion promotes GNU dephosphorylation and PNG kinase activation to regulate translation. The critical regulatory effect of phosphorylation is shown by replacement in the oocyte with a phosphorylation-resistant form of GNU, which promotes PNG-GNU complex formation, elevation of Cyclin B, and meiotic defects consistent with premature PNG activation. After PNG activation GNU is destabilized, thus inactivating PNG. This short-lived burst in kinase activity links development with maternal mRNA translation and ensures irreversibility of the oocyteto- embryo transition.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Attribution 4.0 International (CC BY 4.0)
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DOI of Published Version
https://doi.org/10.7554/eLife.22219
