Class II MHC Self-Antigen Presentation in Human B and T Lymphocytes
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Costantino-2012-Class II MHC self-an.pdf
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Author(s)
Costantino, Cristina Maria
Hafler, David A.
Spooner, Eric
Ploegh, Hidde
Date Issued
January 2012
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Costantino, Cristina Maria et al. “Class II MHC Self-Antigen Presentation in Human B and T Lymphocytes.” Ed. Matthew L. Albert. PLoS ONE 7.1 (2012): e29805. Web. 27 June 2012.
Version
Final published version
Abstract
Human CD4[superscript +] T cells process and present functional class II MHC-peptide complexes, but the endogenous peptide repertoire of these non-classical antigen presenting cells remains unknown. We eluted and sequenced HLA-DR-bound self-peptides presented by CD4[superscript +] T cells in order to compare the T cell-derived peptide repertoire to sequences derived from genetically identical B cells. We identified several novel epitopes derived from the T cell-specific proteome, including fragments of CD4 and IL-2. While these data confirm that T cells can present peptides derived from the T-cell specific proteome, the vast majority of peptides sequenced after elution from MHC were derived from the common proteome. From this pool, we identified several identical peptide epitopes in the T and B cell repertoire derived from common endogenous proteins as well as novel endogenous epitopes with promiscuous binding. These findings indicate that the endogenous HLA-DR-bound peptide repertoire, regardless of APC type and across MHC isotype, is largely derived from the same pool of self-protein.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0029805
