Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance
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Boyden_Transgenic mice.pdf
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1.31 MB
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Author(s)
Madisen, Linda
Garner, Aleena R.
Shimaoka, Daisuke
Li, Lu
van der Bourg, Alexander
Niino, Yusuke
Egolf, Ladan
Monetti, Claudio
Gu, Hong
Mills, Maya
Date Issued
March 2015
Journal
Neuron
Publisher
Elsevier B.V.
Citation
Madisen, Linda, Aleena R. Garner, Daisuke Shimaoka, Amy S. Chuong, Nathan C. Klapoetke, Lu Li, Alexander van der Bourg, et al. "Transgenic Mice for Intersectional Targeting of Neural Sensors and Effectors with High Specificity and Performance." Neuron vol.85, issue 5 (4 March 2015), pp. 942–958.
Version
Author's final manuscript
Abstract
An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity.
Description
available in PMC 2016 March 04
MIT Department
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.neuron.2015.02.022
