Neuron Specific Rab4 Effector GRASP-1 Coordinates Membrane Specialization and Maturation of Recycling Endosomes
Name
Hoogenraad-2010-Neuron Specific Rab4.pdf
Size
11.07 MB
Format
Adobe PDF
Checksum (MD5)
5e4e725ecc84af51624a87dacbf416ed
Author(s)
van der Sluijs, Peter
Kapitein, Lukas C.
Jaarsma, Dick
Rehmann, Holger
Klumperman, Judith
Heck, Albert J. R.
Monti, Maria
Govers, Roland
Oorschot, Viola
Dortland, Bjorn R.
Date Issued
January 2010
Journal
PLoS Biology
Publisher
Public Library of Science
Citation
Hoogenraad, Casper C. et al. “Neuron Specific Rab4 Effector GRASP-1 Coordinates Membrane Specialization and Maturation of Recycling Endosomes.” PLoS Biol 8.1 (2010): e1000283. © 2010 Hoogenraad et al.
Version
Final published version
Abstract
The endosomal pathway in neuronal dendrites is essential for membrane receptor trafficking and proper synaptic function and plasticity. However, the molecular mechanisms that organize specific endocytic trafficking routes are poorly understood. Here, we identify GRIP-associated protein-1 (GRASP-1) as a neuron-specific effector of Rab4 and key component of the molecular machinery that coordinates recycling endosome maturation in dendrites. We show that GRASP-1 is necessary for AMPA receptor recycling, maintenance of spine morphology, and synaptic plasticity. At the molecular level, GRASP-1 segregates Rab4 from EEA1/Neep21/Rab5-positive early endosomal membranes and coordinates the coupling to Rab11-labelled recycling endosomes by interacting with the endosomal SNARE syntaxin 13. We propose that GRASP-1 connects early and late recycling endosomal compartments by forming a molecular bridge between Rab-specific membrane domains and the endosomal SNARE machinery. The data uncover a new mechanism to achieve specificity and directionality in neuronal membrane receptor trafficking.
MIT Department
Picower Institute for Learning and Memory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
http://dx.doi.org/10.1371/journal.pbio.1000283
