Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria monocytogenes
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Gertler_Lamellipodin Is Important.pdf
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Author(s)
Wang, Jiahui
King, Jane E.
Goldrick, Marie
Lowe, Martin
Roberts, Ian S.
Gertler, Frank
Date Issued
July 2015
Journal
Infection and Immunity
Publisher
American Society for Microbiology (ASM)
Citation
Wang, Jiahui, Jane E. King, Marie Goldrick, Martin Lowe, Frank B. Gertler, and Ian S. Roberts. “Lamellipodin Is Important for Cell-to-Cell Spread and Actin-Based Motility in Listeria Monocytogenes.” Edited by A. Camilli. Infect. Immun. 83, no. 9 (July 13, 2015): 3740–3748.
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Final published version
Abstract
Listeria monocytogenes is a foodborne pathogen capable of invading a broad range of cell types and replicating within the host cell cytoplasm. This paper describes the colocalization of host cell lamellipodin (Lpd) with intracellular L. monocytogenes detectable 6 h postinfection of epithelial cells. The association was mediated via interactions between both the peckstrin homology (PH) domain in Lpd and phosphatidylinositol (3,4)-bisphosphate [PI(3,4)P2] on the bacterial surface and by interactions between the C-terminal EVH1 (Ena/VASP [vasodilator-stimulated phosphoprotein] homology domain 1) binding domains of Lpd and the host VASP (vasodilator-stimulated phosphoprotein) recruited to the bacterial cell surface by the listerial ActA protein. Depletion of Lpd by short interfering RNA (siRNA) resulted in reduced plaque size and number, indicating a role for Lpd in cell-to-cell spread. In contrast, overexpression of Lpd resulted in an increase in the number of L. monocytogenes-containing protrusions (listeriopods). Manipulation of the levels of Lpd within the cell also affected the intracellular velocity of L. monocytogenes, with a reduction in Lpd corresponding to an increase in intracellular velocity. These data, together with the observation that Lpd accumulated at the interface between the bacteria and the developing actin tail at the initiation of actin-based movement, indicate a possible role for Lpd in the actin-based movement and the cell-to-cell spread of L. monocytogenes.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1128/iai.00193-15
