Integration of Solid-State Nanopores in Microfluidic Networks via Transfer Printing of Suspended Membranes
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Author(s)
Jain, Tarun
Guerrero, Ricardo Jose S.
Aguilar, Carlos A.
Karnik, Rohit
Date Issued
January 2013
Journal
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Citation
Jain, Tarun, Ricardo Jose S. Guerrero, Carlos A. Aguilar, and Rohit Karnik. “Integration of Solid-State Nanopores in Microfluidic Networks via Transfer Printing of Suspended Membranes.” Analytical Chemistry 85, no. 8 (April 16, 2013): 3871–3878.
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Author's final manuscript
Abstract
Solid-state nanopores have emerged as versatile single-molecule sensors for applications including DNA sequencing, protein unfolding, micro-RNA detection, label-free detection of single nucleotide polymorphisms, and mapping of DNA-binding proteins involved in homologous recombination. While machining nanopores in dielectric membranes provides nanometer-scale precision, the rigid silicon support for the membrane contributes capacitive noise and limits integration with microfluidic networks for sample preprocessing. Herein, we demonstrate a technique to directly transfer solid-state nanopores machined in dielectric membranes from a silicon support into a microfluidic network. The resulting microfluidic-addressable nanopores can sense single DNA molecules at high bandwidths and with low noise, owing to significant reductions in membrane capacitance. This strategy will enable large-scale integration of solid-state nanopores with microfluidic upstream and downstream processing and permit new functions with nanopores such as complex manipulations for multidimensional analysis and parallel sensing in two and three-dimensional architectures.
MIT Department
Lincoln Laboratory
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ac302972c
