Structure and function of ClpXP, a AAA+ proteolytic machine powered by probabilistic ATP hydrolysis
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nihms-1863995.pdf
Description
Accepted version
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1.19 MB
Format
Adobe PDF
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Author(s)
Sauer, Robert T
Fei, Xue
Bell, Tristan A
Baker, Tania A
Date Issued
2022
Journal
Critical Reviews in Biochemistry and Molecular Biology
Publisher
Informa UK Limited
Citation
Sauer, Robert T, Fei, Xue, Bell, Tristan A and Baker, Tania A. 2022. "Structure and function of ClpXP, a AAA+ proteolytic machine powered by probabilistic ATP hydrolysis." Critical Reviews in Biochemistry and Molecular Biology, 57 (2).
Version
Author's final manuscript
Abstract
ClpXP is an archetypical AAA+ protease, consisting of ClpX and ClpP. ClpX is an ATP-dependent protein unfoldase and polypeptide translocase, whereas ClpP is a self-compartmentalized peptidase. ClpXP is currently the only AAA+ protease for which high-resolution structures exist, the molecular basis of recognition for a protein substrate is understood, extensive biochemical and genetic analysis have been performed, and single-molecule optical trapping has allowed direct visualization of the kinetics of substrate unfolding and translocation. In this review, we discuss our current understanding of ClpXP structure and function, evaluate competing sequential and probabilistic mechanisms of ATP hydrolysis, and highlight open questions for future exploration.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1080/10409238.2021.1979461
