Stabilization of Therapeutic Proteins
Name
MEBCS028.pdf
Size
1.62 MB
Format
Adobe PDF
Checksum (MD5)
1e4d188775a1ef1a5a7f5fbbbf494e42
Author(s)
Chu, Jhih-Wei
Yin, Jin
Mazyar, Oleg
Goh, Lin-Tang
Yap, Miranda G.S.
Wang, Daniel I.C.
Trout, Bernhardt L.
Date Issued
January 2003
Series/Report no.
Molecular Engineering of Biological and Chemical Systems (MEBCS);
Abstract
We present results of molecular simulations, quantum mechanical calculations, and experimental data aimed towards the rational design of solvent formulations. In particular, we have found that the rate limitation of oxidation of methionine groups is determined by the breaking of O-O bonds in hydrogen peroxide, not by the rate of acidic catalysis as previously thought. We have used this understanding to design molecular level parameters which are correlated to experimental data. Rate data has been determined both for G-CSF and for hPTH(1-34).
Subjects
protein stabilization
excipients
molecular simulations
kinetics
Persistent DSpace Link
