Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements
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Karnik_Enhanced discrimination.pdf
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1.63 MB
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Author(s)
Sen, Yi-Heng
Jain, Tarun Kumar
Aguilar, Carlos A.
Karnik, Rohit
Date Issued
February 2012
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry, The
Citation
Sen, Yi-Heng, Tarun Jain, Carlos A. Aguilar, and Rohit Karnik. Enhanced Discrimination of DNA Molecules in Nanofluidic Channels through Multiple Measurements. Lab on a Chip 12(6): 1094, 2012.
Version
Author's final manuscript
Abstract
Nanofluidic sensing elements have been the focus of recent experiments for numerous applications ranging from nucleic acid fragment sizing to single-molecule DNA sequencing. These applications critically rely on high measurement fidelity, and methods to increase resolution are required. Herein, we describe fabrication and testing of a nanochannel device that enhances measurement resolution by performing multiple measurements (>100) on single DNA molecules. The enhanced measurement resolution enabled length discrimination between a mixture of λ-DNA (48.5 kbp) and T7 DNA (39.9 kbp) molecules, which were detected as transient current changes during translocation of the molecules through the nanochannel. As long DNA molecules are difficult to resolve quickly and with high fidelity with conventional electrophoresis, this approach may yield potentially portable, direct electrical sizing of DNA fragments with high sensitivity and resolution.
Description
Author Manuscript 2013 March 21.
MIT Department
Lincoln Laboratory
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1039/c2lc20771k
