Porous silicon–graphene oxide core–shell nanoparticles for targeted delivery of siRNA to the injured brain
Name
nihms951198.pdf
Description
Accepted version
Size
1.71 MB
Format
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Checksum (MD5)
3850872a1cdd2be32f9a903991cbcfd8
Author(s)
Joo, Jinmyoung
Kwon, Ester J
Kang, Jinyoung
Skalak, Matthew
Anglin, Emily J
Mann, Aman P
Ruoslahti, Erkki
Bhatia, Sangeeta N
Sailor, Michael J
Date Issued
2016
Journal
Nanoscale Horiz.
Publisher
Royal Society of Chemistry (RSC)
Version
Author's final manuscript
Abstract
© 2016 The Royal Society of Chemistry. We report the synthesis, characterization, and assessment of a nanoparticle-based RNAi delivery platform that protects siRNA payloads against nuclease-induced degradation and efficiently delivers them to target cells. The nanocarrier is based on biodegradable mesoporous silicon nanoparticles (pSiNPs), where the voids of the nanoparticles are loaded with siRNA and the nanoparticles are encapsulated with graphene oxide nanosheets (GO-pSiNPs). The graphene oxide encapsulant delays release of the oligonucleotide payloads in vitro by a factor of 3. When conjugated to a targeting peptide derived from the rabies virus glycoprotein (RVG), the nanoparticles show 2-fold greater cellular uptake and gene silencing. Intravenous administration of the nanoparticles into brain-injured mice results in substantial accumulation specifically at the site of injury.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1039/C6NH00082G
