Remodeling of a delivery complex allows ClpS-mediated degradation of N-degron substrates
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Author(s)
Baker, Tania
Rivera Rivera, Izarys
Roman-Hernandez, Giselle
Sauer, Robert T.
Date Issued
September 2014
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Rivera-Rivera, Izarys et al. “Remodeling of a Delivery Complex Allows ClpS-Mediated Degradation of N-Degron Substrates.” Proceedings of the National Academy of Sciences 111.37 (2014): E3853–E3859. © 2014 National Academy of Sciences
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Final published version
Abstract
The ClpS adaptor collaborates with the AAA+ ClpAP protease to recognize and degrade N-degron substrates. ClpS binds the substrate N-degron and assembles into a high-affinity ClpS-substrate-ClpA complex, but how the N-degron is transferred from ClpS to the axial pore of the AAA+ ClpA unfoldase to initiate degradation is not known. Here we demonstrate that the unstructured N-terminal extension (NTE) of ClpS enters the ClpA processing pore in the active ternary complex. We establish that ClpS promotes delivery only in cis, as demonstrated by mixing ClpS variants with distinct substrate specificity and either active or inactive NTE truncations. Importantly, we find that ClpA engagement of the ClpS NTE is crucial for ClpS-mediated substrate delivery by using ClpS variants carrying “blocking” elements that prevent the NTE from entering the pore. These results support models in which enzymatic activity of ClpA actively remodels ClpS to promote substrate transfer, and highlight how ATPase/motor activities of AAA+ proteases can be critical for substrate selection as well as protein degradation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1073/pnas.1414933111
