A contactless electrical stimulator: application to fabricate functional skeletal muscle tissue
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10544_2012_Article_9692.pdf
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Author(s)
Ahadian, Samad
Ramón-Azcón, Javier
Ostrovidov, Serge
Kaji, Hirokazu
Ino, Kosuke
Shiku, Hitoshi
Matsue, Tomokazu
Camci-Unal, Gulden
Khademhosseini, Alireza
Date Issued
September 2012
Journal
Biomedical Microdevices
Publisher
Springer US
Citation
Kishida, Masako, and Richard D. Braatz. “Ellipsoidal Bounds on State Trajectories for Discrete-Time Systems with Linear Fractional Uncertainties.” Optimization and Engineering 16.4 (2015): 695–711.
Version
Author's final manuscript
Abstract
Engineered skeletal muscle tissues are ideal candidates for applications in drug screening systems, bio-actuators, and as implantable constructs in tissue engineering. Electrical field stimulation considerably improves the differentiation of muscle cells to muscle myofibers. Currently used electrical stimulators often use direct contact of electrodes with tissue constructs or their culture medium, which may cause hydrolysis of the culture medium, joule heating of the medium, contamination of the culture medium due to products of electrodes corrosion, and surface fouling of electrodes. Here, we used an interdigitated array of electrodes combined with an isolator coverslip as a contactless platform to electrically stimulate engineered muscle tissue, which eliminates the aforementioned problems. The effective stimulation of muscle myofibers using this device was demonstrated in terms of contractile activity and higher maturation as compared to muscle tissues without applying the electrical field. Due to the wide array of potential applications of electrical stimulation to two- and three-dimensional (2D and 3D) cell and tissue constructs, this device could be of great interest for a variety of biological applications as a tool to create noninvasive, safe, and highly reproducible electric fields.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1007/s10544-012-9692-1
