Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging
Name
nihms979552.pdf
Description
Accepted version
Size
1.33 MB
Format
Adobe PDF
Checksum (MD5)
0db6dd688c932349dcbbf23f4fbc5297
Author(s)
Chyan, Wen
Raines, Ronald T
Date Issued
June 2018
Journal
ACS Chemical Biology
Publisher
American Chemical Society (ACS)
Citation
Chyan, Wen and Ronald T. Raines. "Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging." ACS Chemical Biology 13, 7 (June 2018): 1810-1823 © 2018 American Chemical Society
Version
Author's final manuscript
Abstract
Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology. Those probes that respond to enzymatic catalysis illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. Here, we review recent advances in enzyme-activated fluorogenic probes for biological imaging. We organize our survey by enzyme classification, with emphasis on fluorophore masking strategies, modes of enzymatic activation, and the breadth of current and future applications. Key challenges such as probe selectivity and spectroscopic requirements are described alongside therapeutic, diagnostic, and theranostic opportunities. Keywords: cells; peptides and proteins; imaging probes; fluorescence; probes
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/acschembio.8b00371
