A research-inspired laboratory sequence investigating acquired drug resistance
Name
BAMBED 2010_Taylor_Fortune_Drennan.pdf
Size
1.37 MB
Format
Adobe PDF
Checksum (MD5)
8f48f26365f570116c7aefa865313ded
Author(s)
Taylor, Elizabeth Vogel
Fortune, Jennifer A.
Drennan, Catherine L
Date Issued
August 2010
Journal
Biochemistry and Molecular Biology Education
Publisher
John Wiley & Sons
Citation
Taylor, E. V., Fortune, J. A. and Drennan, C. L. (2010), A research-inspired laboratory sequence investigating acquired drug resistance. Biochemistry and Molecular Biology Education, 38: 247–252. doi: 10.1002/bmb.20384
Version
Author's final manuscript
Abstract
Here, we present a six-session laboratory exercise designed to introduce students to standard biochemical techniques in the context of investigating a high impact research topic, acquired resistance to the cancer drug Gleevec. Students express a Gleevec-resistant mutant of the Abelson tyrosine kinase domain, the active domain of an oncogenic protein implicated in chronic myelogenous leukemia, and investigate the kinase activity of wild type and mutant enzyme in the presence of two cancer drugs. Techniques covered include protein expression, purification, and gel analysis, kinase activity assays, and protein structure viewing. The exercises provide students with a hands-on understanding of the impact of biochemistry on human health, and demonstrate their potential as the next generation of investigators.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1002/bmb.20384
