Removing human immunodeficiency virus (HIV) from human blood using immobilized heparin
Name
Removing human immunodeficiency virus from human blood using immobilized heparin-20111126 revised.pdf
Size
246.65 KB
Format
Adobe PDF
Checksum (MD5)
b427f70ef744d4183dd6c4dee5f50a2f
Author(s)
Nassar, Roger A.
Browne, Edward P.
Chen, Jianzhu
Klibanov, Alexander M.
Date Issued
December 2011
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Citation
Nassar, Roger A. et al. “Removing Human Immunodeficiency Virus (HIV) from Human Blood Using Immobilized Heparin.” Biotechnology Letters 34.5 (2011): 853–856.
Version
Author's final manuscript
Abstract
Heparin covalently attached to a water-insoluble resin suspended in HIV-infected aqueous buffer or whole blood captures the virus; subsequent physical separation of the immobilized heparin reduced the viral titers by over 80 and 50%, respectively. The detoxification concept has been validated by both circulating an HIV-1 solution through a column packed with the heparin–sepharose beads and successively mixing an HIV-1 solution with fresh beads.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1007/s10529-011-0840-0
