Solid-State NMR Characterization of Gas Vesicle Structure
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Sivertsen-2010-Solid-State NMR Char.pdf
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Author(s)
Sivertsen, Astrid C.
Bayro, Marvin J.
Belenky, Marina
Griffin, Robert Guy
Herzfeld, Judith
Date Issued
September 2010
Journal
Biophysical Journal
Publisher
Elsevier B.V.
Citation
Sivertsen, Astrid C., Marvin J. Bayro, Marina Belenky, Robert G. Griffin, and Judith Herzfeld. “Solid-State NMR Characterization of Gas Vesicle Structure.” Biophysical Journal 99, no. 6 (September 2010): 1932–1939. © 2010 Biophysical Society.
Version
Final published version
Abstract
Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-α-β-β-α-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.bpj.2010.06.041
