Biosensor development for single-cell detection of glucuronate
Name
kuad013.pdf
Description
Published version
Size
1.24 MB
Format
Adobe PDF
Checksum (MD5)
aed88597b314e634ab17abb7c307368e
Author(s)
Nash, Jennifer Kaczmarek
Prather, Kristala LJ
Date Issued
June 16, 2023
Journal
Journal of Industrial Microbiology and Biotechnology
Publisher
Oxford University Press
Citation
Jennifer Kaczmarek Nash, Kristala L J Prather, Biosensor development for single-cell detection of glucuronate, Journal of Industrial Microbiology and Biotechnology, Volume 50, Issue 1, 2023, kuad013.
Version
Final published version
Abstract
Recent work in biosensors has shown promise to enable high throughput searches through large genetic libraries. However, just as physiological limitations and lack of in-depth mechanistic knowledge can prevent us from achieving high titers in microbial systems; similar roadblocks can appear in the application of biosensors. Here, we characterized a previously developed transcription-factor (ExuR) based galacturonate biosensor for its other cognate ligand, glucuronate. Though we saw an ideal response to glucuronate from the biosensor in controlled and ideal experimental circumstances, these results began to deviate from a well-behaved system when we explored the application of the sensor to different MIOX homologs. Through modifications to circuit architecture and culture conditions, we were able to decrease this variation and use these more optimal conditions to apply the biosensor for the separation of two closely related MIOX homologs.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1093/jimb/kuad013
