Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics
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c8lc00250a.pdf
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5.2 MB
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Checksum (MD5)
87035a8ac3b317c13425714f5b1915b3
Author(s)
Kwon, Taehong
Yao, Rujie
Hamel, Jean-Francois P
Han, Jongyoon
Date Issued
July 2018
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Citation
Kwon, Taehong et al. “Continuous Removal of Small Nonviable Suspended Mammalian Cells and Debris from Bioreactors Using Inertial Microfluidics.” Lab on a Chip (July 2018) © 2018 Royal Society of Chemistry
Version
Final published version
Abstract
Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Label-free microfluidic cell separation devices based on dielectrophoresis, acoustophoresis, and deterministic lateral displacement are used to remove nonviable cells. However, their volumetric throughputs and test cell concentrations are generally too low to be useful in typical bioreactors in biomanufacturing. In this study, we demonstrate the efficient removal of small (<10 μm) nonviable cells from bioreactors while maintaining viable cells using inertial microfluidic cell sorting devices and characterize their performance. Despite the size overlap between viable and nonviable cell populations, the devices demonstrated 3.5-28.0% dead cell removal efficiency with 88.3-83.6% removal purity as well as 97.8-99.8% live cell retention efficiency at 4 million cells per mL with 80% viability. Cascaded and parallel configurations increased the cell concentration capacity (10 million cells per mL) and volumetric throughput (6-8 mL min-1). The system can be used for the removal of small nonviable cells from a cell suspension during continuous perfusion cell culture operations.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution-NonCommercial 3.0 Unported
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DOI of Published Version
https://doi.org/10.1039/c8lc00250a
