On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening
Name
Rohde-2009-On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo druggenetic screening.pdf
Size
546.83 KB
Format
Adobe PDF
Checksum (MD5)
298c6ce81d2f3200a25154332a1a46ac
Author(s)
Yanik, Mehmet Fatih
Rohde, Christopher Benjamin
Gilleland, Cody Lee
Samara, Chrysanthi
Date Issued
May 2009
Journal
IEEE/NIH Life Science Systems and Applications Workshop, 2009. LiSSA 2009
Publisher
Institute of Electrical and Electronics Engineers
Citation
Rohde, C.B. et al. “On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening.” Life Science Systems and Applications Workshop, 2009. LiSSA 2009. IEEE/NIH. 2009. 52-55. © 2009 IEEE.
Version
Final published version
Abstract
Techniques for rapid and automated small-animal manipulation and immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multicellular organisms. We present a suite of technologies for complex high-throughput whole-animal genetic and drug screens. We demonstrate a high-speed microfluidic sorter that can isolate and immobilize 'Caenorhabditis elegans' in a well-defined geometry, an integrated chip containing individually addressable screening chambers for incubation and exposure of individual animals to biochemical compounds, and a device for delivery of compound libraries in standard multiwell plates to microfluidic devices. The immobilization stability obtained by these devices is comparable to that of chemical anesthesia and the immobilization process does not affect lifespan, progeny production, or other aspects of animal health. The high-stability enables the use of a variety of key optical techniques. We use this to demonstrate femtosecond-laser nanosurgery and three-dimensional multiphoton microscopy to study neural regeneration at sub-cellular resolution in vivo.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1109/LISSA.2009.4906707
