Tissue sampling array for tumor marker profiling
Name
66911598-MIT.pdf
Description
Full printable version
Size
13.03 MB
Format
Adobe PDF
Checksum (MD5)
1bc88207fd1bc7f8679bfeb9b3ad30b7
Author(s)
Manoosingh, Sasha R. (Sasha Rianne), 1979-
Advisor(s)
Ian W. Hunter.
Date Issued
2005
Publisher
Massachusetts Institute of Technology
Abstract
The rise of prostate cancer diagnoses in the early 1990's due to better detection techniques and the recent advances in the clinical management of the disease have required better technologies for detection and monitoring disease status. The focus of my research at the Bioinstrumentation Laboratory was to design, fabricate and test an instrument for the preparation of excised frozen tissue from prostate cancer tumors in patients for conformal mapping of the sampling points to the local tissue geometry. This instrument was designed specifically for use on frozen samples that have been archived and the work involves solving issues inherent in extracting information from frozen tissue without denaturing DNA, RNA and proteins. Isolation of the nucleic acids from the bulk tissue and real time PCR amplification of a 152 bp segment of the androgen receptor was used to verify that the tissue was extracted successfully using the device. It is the intention of this work and others like it that quick and efficient mapping technologies will come into widespread use in predicting tumor size and growth rates thereby improving cancer patient diagnosis and treatment.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005.
Includes bibliographical references (leaves 76-77).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
Persistent DSpace Link
