Engineering E. coli–E. coli cocultures for production of muconic acid from glycerol
Name
12934_2015_Article_319.pdf
Size
1.14 MB
Format
Adobe PDF
Checksum (MD5)
93355654d9e7dfed41155bbbf73d245e
Author(s)
Zhang, Haoran
Li, Zhengjun
Stephanopoulos, Gregory
Pereira, Brian J.
Date Issued
September 2015
Journal
Microbial Cell Factories
Publisher
BioMed Central Ltd.
Citation
Zhang, Haoran, Zhengjun Li, Brian Pereira, and Gregory Stephanopoulos. "Engineering E. coli–E. coli cocultures for production of muconic acid from glycerol." Microbial Cell Factories. 2015 Sep 15;14(1):134.
Version
Final published version
Abstract
Background
cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of the E. coli–E. coli coculture system to use a single renewable carbon source, glycerol, for the production of value-added product cis, cis-muconic acid.
Results
Two coculture engineering strategies were investigated. In the first strategy, an E. coli strain containing the complete biosynthesis pathway was co-cultivated with another E. coli strain containing only a heterologous intermediate-to-product biosynthetic pathway. In the second strategy, the upstream and downstream pathways were accommodated in two separate E. coli strains, each of which was dedicated to one portion of the biosynthesis process. Compared with the monoculture approach, both coculture engineering strategies improved the production significantly. Using a batch bioreactor, the engineered coculture achieved a 2 g/L muconic acid production with a yield of 0.1 g/g.
Conclusions
Our results demonstrate that coculture engineering is a viable option for producing muconic acid from glycerol. Moreover, microbial coculture systems are shown to have the potential for converting single carbon source to value-added products.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/s12934-015-0319-0
