Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice
Name
Chen-2012-Imaging Neural Activ.pdf
Size
2.08 MB
Format
Adobe PDF
Checksum (MD5)
7f59292f404f39cdf9eb958044ef3750
Author(s)
Chen, Qian
Cichon, Joseph
Wang, Wenting
Qiu, Li
Lee, Seok-Jin R.
Campbell, Nolan R.
DeStefino, Nicholas
Fu, Zhanyan
Yasuda, Ryohei
Looger, Loren L.
Date Issued
October 2012
Journal
Neuron
Publisher
Elsevier
Citation
Chen, Qian, Joseph Cichon, Wenting Wang, Li Qiu, Seok-Jin R. Lee, Nolan R. Campbell, Nicholas DeStefino, et al. “Imaging Neural Activity Using Thy1-GCaMP Transgenic Mice.” Neuron 76, no. 2 (October 2012): 297–308. © 2012 Elsevier Inc.
Version
Final published version
Abstract
The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Picower Institute for Learning and Memory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.neuron.2012.07.011
