HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology
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fncir-12-00041.pdf
Size
2.34 MB
Format
Adobe PDF
Checksum (MD5)
925dfd5b0a3f8a0981436dee0871f166
Author(s)
Delcasso, Sebastien
Denagamage, Sachira
Britton, Zelie
Graybiel, Ann M
Date Issued
May 2018
Journal
Frontiers in Neural Circuits
Publisher
Frontiers Research Foundation
Citation
Delcasso, Sebastien, Sachira Denagamage, Zelie Britton, and Ann M. Graybiel. “HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology.” Frontiers in Neural Circuits 12 (May 16, 2018).
Version
Final published version
Abstract
Understanding the neural mechanisms underlying human cognition and determining the causal factors for the development of brain pathologies are among the greatest challenges for society. Electrophysiological recordings offer remarkable observations of brain activity as they provide highly precise representations of information coding in both temporal and spatial domains. With the development of genetic tools over the last decades, mice have been a key model organism in neuroscience. However, conducting chronic in vivo electrophysiology in awake, behaving mice remains technically challenging, and this difficulty prevents many research teams from acquiring critical recordings in their mouse models. Behavioral training, implant fabrication, brain surgery, data acquisition and data analysis are all required steps that must be mastered in order to perform cutting-edge experiments in systems neuroscience. Here, we present a new method that simplifies the construction of a drivable and multi-task electrophysiological recording implant without loss of flexibility and recording power. The hybrid-drive combining optogenetics, pharmacology and electrophysiology (HOPE) can support up to 16 tetrodes, attached to a single drive mechanism, organized in two bundles of eight tetrodes, allowing recordings in two different mouse brain regions simultaneously with two optical fibers for optogenetic manipulation or two injection cannulas for drug-delivery experiments. Because it can be printed with a latest-generation desktop 3D printer, the production cost is low compared to classical electrophysiology implants, and it can be built within a few hours. The HOPE implant is also reconfigurable to specific needs as it has been created in a computer-aided design (CAD) software and all the files used for its construction are open-source.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.3389/fncir.2018.00041
