Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices
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Author(s)
Weitz, David A.
Prakadan, Sanjay
Shalek, Alexander K
Date Issued
April 2017
Journal
Nature Reviews Genetics
Publisher
Nature Publishing Group
Citation
Prakadan, Sanjay M. et al. “Scaling by Shrinking: Empowering Single-Cell ‘Omics’ with Microfluidic Devices.” Nature Reviews Genetics 18, 6 (April, 2017): 345–361 © 2017 Macmillan Publishers Limited, part of Springer Nature
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Author's final manuscript
Abstract
All rights reserved. Recent advances in cellular profiling have demonstrated substantial heterogeneity in the behaviour of cells once deemed 'identical', challenging fundamental notions of cell 'type' and 'state'. Not surprisingly, these findings have elicited substantial interest in deeply characterizing the diversity, interrelationships and plasticity among cellular phenotypes. To explore these questions, experimental platforms are needed that can extensively and controllably profile many individual cells. Here, microfluidic structures-whether valve-, droplet- or nanowell-based-have an important role because they can facilitate easy capture and processing of single cells and their components, reducing labour and costs relative to conventional plate-based methods while also improving consistency. In this article, we review the current state-of-the-art methodologies with respect to microfluidics for mammalian single-cell 'omics' and discuss challenges and future opportunities.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Chemistry
Ragon Institute of MGH, MIT and Harvard
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DOI of Published Version
https://doi.org/10.1038/NRG.2017.15
