Telomerase expression marks transitional growth-associated skeletal progenitor/stem cells: mTert marks skeletal progenitor/stem cells
Name
stem.3318.pdf
Description
Published version
Size
10.29 MB
Format
Adobe PDF
Checksum (MD5)
9d44b2a1355c7429840e849432c35285
Author(s)
Carlone, Diana L
Riba-Wolman, Rebecca D
Deary, Luke T
Tovaglieri, Alessio
Jiang, Lijie
Ambruzs, Dana M
Mead, Benjamin E
Shah, Manasvi S
Lengner, Christopher J
Jaenisch, Rudolf
Date Issued
2021
Journal
Stem Cells
Publisher
Wiley
Version
Final published version
Abstract
©2021 The Authors. Stem Cells published by Wiley Periodicals LLC on behalf of AlphaMed Press. Skeletal progenitor/stem cells (SSCs) play a critical role in postnatal bone growth and maintenance. Telomerase (Tert) activity prevents cellular senescence and is required for maintenance of stem cells in self-renewing tissues. Here we investigated the role of mTert-expressing cells in postnatal mouse long bone and found that mTert expression is enriched at the time of adolescent bone growth. mTert-GFP+ cells were identified in regions known to house SSCs, including the metaphyseal stroma, growth plate, and the bone marrow. We also show that mTert-expressing cells are a distinct SSC population with enriched colony-forming capacity and contribute to multiple mesenchymal lineages, in vitro. In contrast, in vivo lineage-tracing studies identified mTert+ cells as osteochondral progenitors and contribute to the bone-forming cell pool during endochondral bone growth with a subset persisting into adulthood. Taken together, our results show that mTert expression is temporally regulated and marks SSCs during a discrete phase of transitional growth between rapid bone growth and maintenance.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/stem.3318
