Opportunities in metabolic engineering to facilitate scalable alkaloid production
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Prather-Opportunities in metabolic engineering.pdf
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199.52 KB
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Author(s)
Leonard, Effendi
Runguphan, Weerawat
O'Connor, Sarah Ellen
Prather, Kristala L. Jones
Date Issued
April 2009
Journal
Nature Chemical Biology
Publisher
Nature Publishing Group
Citation
Leonard, Effendi et al. “Opportunities in metabolic engineering to facilitate scalable alkaloid production.” Nature Chemical Biology 5.5 (2009): 292-300.
Version
Author's final manuscript
Abstract
Numerous drugs and drug precursors in the current pharmacopoeia originate from plant sources. The limited yield of some bioactive compounds in plant tissues, however, presents a significant challenge for large-scale drug development. Metabolic engineering has facilitated the development of plant cell and tissue systems as alternative production platforms that can be scaled up in a controlled environment. Nevertheless, effective metabolic engineering approaches and the predictability of genetic transformations are often obscured due to the myriad cellular complexities. Progress in systems biology has aided the understanding of genome-wide interconnectivities in plant-based systems. In parallel, the bottom-up assembly of plant biosynthetic pathways in microorganisms demonstrated the possibilities of a new means of production. In this Perspective, we discuss the opportunities and challenges of implementing metabolic engineering in various platforms for the synthesis of natural and unnatural plant alkaloids.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/nchembio.160
