Effective use of biosensors for high-throughput library screening for metabolite production
Name
kuab049.pdf
Description
Published version
Size
534.34 KB
Format
Adobe PDF
Checksum (MD5)
86a4747e5502bea9b85099de7fe6b7a7
Author(s)
Kaczmarek, Jennifer A
Prather, Kristala LJ
Date Issued
August 4, 2021
Journal
Journal of Industrial Microbiology and Biotechnology
Publisher
Oxford University Press
Citation
Jennifer A Kaczmarek, Kristala L J Prather, Effective use of biosensors for high-throughput library screening for metabolite production, Journal of Industrial Microbiology and Biotechnology, Volume 48, Issue 9-10, December 2021, kuab049.
Version
Final published version
Abstract
The development of fast and affordable microbial production from recombinant pathways is a challenging endeavor, with targeted improvements difficult to predict due to the complex nature of living systems. To address the limitations in biosynthetic pathways, much work has been done to generate large libraries of various genetic parts (promoters, RBSs, enzymes, etc.) to discover library members that bring about significantly improved levels of metabolite production. To evaluate these large libraries, high throughput approaches are necessary, such as those that rely on biosensors. There are various modes of operation to apply biosensors to library screens that are available at different scales of throughput. The effectiveness of each biosensor-based method is dependent on the pathway or strain to which it is applied, and all approaches have strengths and weaknesses to be carefully considered for any high throughput library screen. In this review, we discuss the various approaches used in biosensor screening for improved metabolite production, focusing on transcription factor-based biosensors.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1093/jimb/kuab049
