A general screening strategy for peptide-based fluorogenic ligands
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Imperiali-A general screening strategy.pdf
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Author(s)
Sainlos, Matthieu
Iskenderian, Wendy S.
Imperiali, Barbara
Date Issued
April 2009
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Sainlos, Matthieu, Wendy S. Iskenderian, and Barbara Imperiali. “A General Screening Strategy for Peptide-Based Fluorogenic Ligands: Probes for Dynamic Studies of PDZ Domain-Mediated Interactions.” Journal of the American Chemical Society 131.19 (2009): 6680–6682.
Version
Author's final manuscript
Abstract
A systematic and general approach for identifying efficient probes for class I PDZ domains based on environment-sensitive chromophores is presented. A series of peptides derived from the C-terminal sequence of Stargazin was first used with PDZ domains of PSD-95 and Shank3 to identify the optimal position and linker length for the 4-DMAP chromophore. The results were applied to well-characterized ligand sequences for each set of domains to generate high affinity probes that retain their native sequence specificity and yield remarkable fluorescence increases upon binding. These probes constitute efficient tools to study the dynamics and regulatory mechanisms of PDZ domain-mediated interactions.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
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DOI of Published Version
https://doi.org/10.1021/ja900371q
