Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states
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s41467-021-27322-4.pdf
Description
Published version
Size
11.93 MB
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Checksum (MD5)
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Author(s)
Song, Hanbing
Weinstein, Hannah NW
Allegakoen, Paul
Wadsworth, Marc H
Xie, Jamie
Yang, Heiko
Castro, Ethan A
Lu, Kevin L
Stohr, Bradley A
Feng, Felix Y
Date Issued
December 2022
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Citation
Song, Hanbing, Weinstein, Hannah NW, Allegakoen, Paul, Wadsworth, Marc H, Xie, Jamie et al. 2022. "Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states." Nature Communications, 13 (1).
Version
Final published version
Abstract
AbstractProstate cancer is the second most common malignancy in men worldwide and consists of a mixture of tumor and non-tumor cell types. To characterize the prostate cancer tumor microenvironment, we perform single-cell RNA-sequencing on prostate biopsies, prostatectomy specimens, and patient-derived organoids from localized prostate cancer patients. We uncover heterogeneous cellular states in prostate epithelial cells marked by high androgen signaling states that are enriched in prostate cancer and identify a population of tumor-associated club cells that may be associated with prostate carcinogenesis. ERG-negative tumor cells, compared to ERG-positive cells, demonstrate shared heterogeneity with surrounding luminal epithelial cells and appear to give rise to common tumor microenvironment responses. Finally, we show that prostate epithelial organoids harbor tumor-associated epithelial cell states and are enriched with distinct cell types and states from their parent tissues. Our results provide diagnostically relevant insights and advance our understanding of the cellular states associated with prostate carcinogenesis.
MIT Department
Ragon Institute of MGH, MIT and Harvard
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Chemistry
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/s41467-021-27322-4
