Planar 2D wireframe DNA origami
Name
sciadv.abn0039.pdf
Description
Published version
Size
933.05 KB
Format
Adobe PDF
Checksum (MD5)
4b4f74a98d02fded5e62ae061cc7695a
Author(s)
Wang, Xiao
Li, Shanshan
Jun, Hyungmin
John, Torsten
Zhang, Kaiming
Fowler, Hannah
Doye, Jonathan PK
Chiu, Wah
Bathe, Mark
Date Issued
2022
Journal
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Wang, Xiao, Li, Shanshan, Jun, Hyungmin, John, Torsten, Zhang, Kaiming et al. 2022. "Planar 2D wireframe DNA origami." Science Advances, 8 (20).
Version
Final published version
Abstract
Two-dimensional (2D) DNA origami is widely used for applications ranging from excitonics to single-molecule biophysics. Conventional, single-layer 2D DNA origami exhibits flexibility and curvature in solution; however, that may limit its suitability as a 2D structural template. In contrast, 2D wireframe DNA origami rendered with six-helix bundle edges offers local control over duplex orientations with enhanced in-plane rigidity. Here, we investigate the 3D structure of these assemblies using cryo–electron microscopy (cryo-EM). 3D reconstructions reveal a high degree of planarity and homogeneity in solution for polygonal objects with and without internal mesh, enabling 10-Å resolution for a triangle. Coarse-grained simulations were in agreement with cryo-EM data, offering molecular structural insight into this class of 2D DNA origami. Our results suggest that these assemblies may be valuable for 2D material applications and geometries that require high structural fidelity together with local control over duplex orientations, rather than parallel duplex assembly.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/SCIADV.ABN0039
