Dynamic single cell imaging of direct reprogramming reveals an early specifying event
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Regev_Dynamic single.pdf
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Author(s)
Smith, Zachary D.
Nachman, Iftach
Regev, Aviv
Meissner, Alexander
Date Issued
May 2010
Journal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Smith, Zachary D et al. “Dynamic Single-cell Imaging of Direct Reprogramming Reveals an Early Specifying Event.” Nature Biotechnology 28.5 (2010): 521–526. Web.
Version
Author's final manuscript
Abstract
The study of induced pluripotency often relies on experimental approaches that average measurements across a large population of cells, the majority of which do not become pluripotent. Here we used high-resolution, time-lapse imaging to trace the reprogramming process over 2 weeks from single mouse embryonic fibroblasts (MEFs) to pluripotency factor–positive colonies. This enabled us to calculate a normalized cell-of-origin reprogramming efficiency that takes into account only the initial MEFs that respond to form reprogrammed colonies rather than the larger number of final colonies. Furthermore, this retrospective analysis revealed that successfully reprogramming cells undergo a rapid shift in their proliferative rate that coincides with a reduction in cellular area. This event occurs as early as the first cell division and with similar kinetics in all cells that form induced pluripotent stem (iPS) cell colonies. These data contribute to the theoretical modeling of reprogramming and suggest that certain parts of the reprogramming process follow defined rather than stochastic steps.
Description
available in PMC 2010 November 1.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/nbt.1632
