Comprehensive analysis of the chromatin landscape in Drosophila melanogaster
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Kellis_Comprehensive analysis.pdf
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1.01 MB
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Author(s)
Ernst, Jason
Kellis, Manolis
Date Issued
December 2010
Journal
Nature
Publisher
Nature Publishing Group
Citation
Kharchenko, Peter V. et al. “Comprehensive Analysis of the Chromatin Landscape in Drosophila Melanogaster.” Nature 471.7339 (2010): 480–485.
Version
Author's final manuscript
Abstract
Chromatin is composed of DNA and a variety of modified histones and non-histone proteins, which have an impact on cell differentiation, gene regulation and other key cellular processes. Here we present a genome-wide chromatin landscape for Drosophila melanogaster based on eighteen histone modifications, summarized by nine prevalent combinatorial patterns. Integrative analysis with other data (non-histone chromatin proteins, DNase I hypersensitivity, GRO-Seq reads produced by engaged polymerase, short/long RNA products) reveals discrete characteristics of chromosomes, genes, regulatory elements and other functional domains. We find that active genes display distinct chromatin signatures that are correlated with disparate gene lengths, exon patterns, regulatory functions and genomic contexts. We also demonstrate a diversity of signatures among Polycomb targets that include a subset with paused polymerase. This systematic profiling and integrative analysis of chromatin signatures provides insights into how genomic elements are regulated, and will serve as a resource for future experimental investigations of genome structure and function.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/nature09725
