PD-L1 is an activation-independent marker of brown adipocytes
Name
s41467-017-00799-8.pdf
Size
2.91 MB
Format
Adobe PDF
Checksum (MD5)
489f665adde3089d59b2605001c5000b
Author(s)
Ingram, Jessica R.
Dougan, Michael
Rashidian, Mohammad
Knoll, Marko
Keliher, Edmund J.
Garrett, Sarah
Garforth, Scott
Blomberg, Olga S.
Espinosa, Camilo
Bhan, Atul
Date Issued
September 2017
Journal
Nature Communications
Publisher
Springer Nature
Citation
Ingram, Jessica R. et al. “PD-L1 Is an Activation-Independent Marker of Brown Adipocytes.” Nature Communications 8, 1 (September 2017): 647 © 2017 The Author(s)
Version
Final published version
Abstract
Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Attribution 4.0 International (CC BY 4.0)
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/S41467-017-00799-8
