Integrin Signaling Switches the Cytoskeletal and Exocytic Machinery that Drives Neuritogenesis
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Gertler_Integrin signaling.pdf
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Author(s)
Gupton, Stephanie L.
Gertler, Frank
Date Issued
May 2010
Journal
Developmental Cell
Publisher
Elsevier
Citation
Gupton, Stephanie L., and Frank B. Gertler. “Integrin Signaling Switches the Cytoskeletal and Exocytic Machinery that Drives Neuritogenesis.” Developmental Cell 18, no. 5 (May 2010): 725-736. Copyright © 2010 Elsevier Inc.
Version
Final published version
Abstract
Neurons establish their unique morphology by elaborating multiple neurites that subsequently form axons and dendrites. Neurite initiation entails significant surface area expansion, necessitating addition to the plasma membrane. We report that regulated membrane delivery coordinated with the actin cytoskeleton is crucial for neuritogenesis and identify two independent pathways that use distinct exocytic and cytoskeletal machinery to drive neuritogenesis. One pathway uses Ena/VASP-regulated actin dynamics coordinated with VAMP2-mediated exocytosis and involves a novel role for Ena/VASP in exocytosis. A second mechanism occurs in the presence of laminin through integrin-dependent activation of FAK and src and uses coordinated activity of the Arp2/3 complex and VAMP7-mediated exocytosis. We conclude that neuritogenesis can be driven by two distinct pathways that differentially coordinate cytoskeletal dynamics and exocytosis. These regulated changes and coordination of cytoskeletal and exocytic machinery may be used in other physiological contexts involving cell motility and morphogenesis.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.devcel.2010.02.017
