A low-cost 3D printed microfluidic bioreactor and imaging chamber for live-organoid imaging
Name
BIOMGB-000015-024105_1.pdf
Description
Published version
Size
6.23 MB
Format
Adobe PDF
Checksum (MD5)
670607fbd720324dbcab2d451b4388fe
Author(s)
Khan, Ikram
Prabhakar, Anil
Delepine, Chloe
Tsang, Hayley
Pham, Vincent
Sur, Mriganka
Date Issued
2021
Journal
Biomicrofluidics
Publisher
AIP Publishing
Citation
Khan, Ikram, Prabhakar, Anil, Delepine, Chloe, Tsang, Hayley, Pham, Vincent et al. 2021. "A low-cost 3D printed microfluidic bioreactor and imaging chamber for live-organoid imaging." Biomicrofluidics, 15 (2).
Version
Final published version
Abstract
Organoids are biological systems grown in vitro and are observed to self-organize into 3D cellular tissues of specific organs. Brain organoids have emerged as valuable models for the study of human brain development in health and disease. Researchers are now in need of improved culturing and imaging tools to capture the in vitro dynamics of development processes in the brain. Here, we describe the design of a microfluidic chip and bioreactor, to enable in situ tracking and imaging of brain organoids on-chip. The low-cost 3D printed microfluidic bioreactor supports organoid growth and provides an optimal imaging chamber for live-organoid imaging, with drug delivery support. This fully isolated design of a live-cell imaging and culturing platform enables long-term live-imaging of the intact live brain organoids as it grows. We can thus analyze their self-organization in a controlled environment with high temporal and spatial resolution.
MIT Department
Picower Institute for Learning and Memory
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1063/5.0041027
