Poly(β-amino ester)–DNA complexes: Time-resolved fluorescence and cellular transfection studies
Name
Anderson_Poly(Beta-amino ester).pdf
Size
1.13 MB
Format
Adobe PDF
Checksum (MD5)
b38abe6ee367dc0f9f7f4eb67297df30
Author(s)
Vuorimaa, Elina
Ketola, Tiia-Maaria
Green, Jordan J.
Lemmetyinen, Helge
Urtti, Arto
Yliperttula, Marjo
Hanzlikova, Martina
Anderson, Daniel Griffith
Langer, Robert S
Date Issued
June 2011
Journal
Journal of Controlled Release
Publisher
Elsevier
Citation
Vuorimaa, Elina, Tiia-Maaria Ketola, Jordan J. Green, Martina Hanzlikova, Helge Lemmetyinen, Robert Langer, Daniel G. Anderson, Arto Urtti, and Marjo Yliperttula. “Poly(β-Amino ester)–DNA Complexes: Time-Resolved Fluorescence and Cellular Transfection Studies.” Journal of Controlled Release 154, no. 2 (September 2011): 171–176.
Version
Author's final manuscript
Abstract
A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(β-amino esters) than for 25 kDa polyethylenimines, yet poly(β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.jconrel.2011.06.016
