Detecting and Understanding the Roles of Nitric Oxide in biology
Name
FinalRevisedMs.pdf
Size
3.51 MB
Format
Adobe PDF
Checksum (MD5)
a5dcb328e8b4ab714c9925c982388941
Author(s)
Tonzetich, Zachary J.
McQuade, Lindsey E.
Lippard, Stephen J.
Date Issued
July 2010
Journal
Inorganic Chemistry
Publisher
American Chemical Society (ACS)
Citation
Tonzetich, Zachary J., Lindsey E. McQuade, and Stephen J. Lippard. “Detecting and Understanding the Roles of Nitric Oxide in Biology.” Inorganic Chemistry 49.14 (2010): 6338–6348.
Version
Author's final manuscript
Abstract
We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to external stimuli. In the other approach, we employ synthetic model complexes of iron−sulfur clusters to probe their reactivity toward nitric oxide as biomimics of the active sites of iron−sulfur proteins. Our studies reveal that NO disassembles the Fe−S clusters to form dinitrosyl iron complexes.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/ic9022757
