Viral reservoir characteristics in lymphoid tissues of HIV-1 elite controllers
Name
197308.2-20251114090222-covered-4fa089109ce452e764bbb8648b44a723.pdf
Description
Published version
Size
2.08 MB
Format
Adobe PDF
Checksum (MD5)
2a47df5dea18c35689c934f8fa3ae1a3
Author(s)
Marzi, Samantha K
Naasz, Chloé M
Carrere, Leah
Capote, Carmen Gasca
Roseto, Isabelle C
Gao, Ce
Cavassini, Matthias
Mastrangelo, Andrea
Lichterfeld, Mathias
Perreau, Matthieu
Date Issued
October 28, 2025
Journal
JCI Insight
Publisher
American Society for Clinical Investigation
Version
Final published version
Abstract
Elite controllers (ECs) maintain undetectable levels of plasma viremia in the absence of treatment, but small reservoirs of replication-competent proviruses persist in the vast majority of these persons. We longitudinally studied paired blood and inguinal lymph node samples (LNMC) from 2 ECs to better characterize distinguishing features of viral reservoir cell dynamics in ECs. In both participants, we observed a 7- to 10-fold lower frequency of intact proviruses in LNMC samples relative to reservoir cells circulating in blood. The landscape of intact proviruses in both tissue compartments was dominated by shared large clones that were frequently integrated in noncoding DNA regions, but the frequency and diversity of intact proviruses was more limited in LNMCs. Of note, over 9–10 years of longitudinal follow-up, a 3- to 18-fold reduction of intact proviruses was observed. Together, these data support the hypothesis that viral reservoirs in EC blood and lymphoid tissues are under strong, likely immune-mediated selection pressure.
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DOI of Published Version
https://doi.org/10.1172/jci.insight.197308
