Synthetic Collagen Hydrogels through Symmetric Self‐Assembly of Small Peptides
Name
Advanced Science - 2023 - Tanrikulu - Synthetic Collagen Hydrogels through Symmetric Self‐Assembly of Small Peptides.pdf
Description
Published version
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4.49 MB
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Adobe PDF
Checksum (MD5)
ae59c8da23450d1540af47f488256580
Author(s)
Tanrikulu, I Caglar
Dang, Lianna
Nelavelli, Lekha
Ellison, Aubrey J
Olsen, Bradley D
Jin, Song
Raines, Ronald T
Date Issued
November 23, 2023
Journal
Advanced Science
Publisher
Wiley
Citation
Tanrikulu, I Caglar, Dang, Lianna, Nelavelli, Lekha, Ellison, Aubrey J, Olsen, Bradley D et al. 2023. "Synthetic Collagen Hydrogels through Symmetric Self‐Assembly of Small Peptides." Advanced Science, 11 (3).
Version
Final published version
Abstract
Animal‐sourced hydrogels, such as collagen, are widely used as extracellular‐matrix (ECM) mimics in tissue engineering but are plagued with problems of reproducibility, immunogenicity, and contamination. Synthetic, chemically defined hydrogels can avoid such issues. Despite the abundance of collagen in the ECM, synthetic collagen hydrogels are extremely rare due to design challenges brought on by the triple‐helical structure of collagen. Sticky‐ended symmetric self‐assembly (SESSA) overcomes these challenges by maximizing interactions between the strands of the triple helix, allowing the assembly of collagen‐mimetic peptides (CMPs) into robust synthetic collagen nanofibers. This optimization, however, also minimizes interfiber contacts. In this work, symmetric association states for the SESSA of short CMPs to probe their increased propensity for interfiber association are modelled. It is found that 33‐residue CMPs not only self‐assemble through sticky ends, but also form hydrogels. These self‐assemblies behave with remarkable consistency across multiple scales and present a clear link between their triple‐helical architecture and the properties of their hydrogels. The results show that SESSA is an effective and robust design methodology that enables the rational design of synthetic collagen hydrogels.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1002/advs.202303228
