Poly(A)-tail profiling reveals an embryonic switch in translational control
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Bartel_Poly(a)-tail.pdf
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Author(s)
Subtelny, Alexander Orest
Eichhorn, Stephen William
Chen, Grace
Sive, Hazel L.
Bartel, David
Date Issued
January 2014
Journal
Nature
Publisher
Nature Publishing Group
Citation
Subtelny, Alexander O., Stephen W. Eichhorn, Grace R. Chen, Hazel Sive, and David P. Bartel. “Poly(A)-Tail Profiling Reveals an Embryonic Switch in Translational Control.” Nature 508, no. 7494 (January 29, 2014): 66–71.
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Author's final manuscript
Abstract
Poly(A) tails enhance the stability and translation of most eukaryotic messenger RNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function. Here we describe poly(A)-tail length profiling by sequencing (PAL-seq) and apply it to measure tail lengths of millions of individual RNAs isolated from yeasts, cell lines, Arabidopsis thaliana leaves, mouse liver, and zebrafish and frog embryos. Poly(A)-tail lengths were conserved between orthologous mRNAs, with mRNAs encoding ribosomal proteins and other ‘housekeeping’ proteins tending to have shorter tails. As expected, tail lengths were coupled to translational efficiencies in early zebrafish and frog embryos. However, this strong coupling diminished at gastrulation and was absent in non-embryonic samples, indicating a rapid developmental switch in the nature of translational control. This switch complements an earlier switch to zygotic transcriptional control and explains why the predominant effect of microRNA-mediated deadenylation concurrently shifts from translational repression to mRNA destabilization.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nature13007
