Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro
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Differentiation of club cells.pdf
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1.45 MB
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Checksum (MD5)
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Author(s)
Zheng, Dahai
Soh, Boon-Seng
Yin, Lu
Hu, Guangan
Chen, Qingfeng
Choi, Hyungwon
Han, Jongyoon
Chow, Vincent T. K.
Chen, Jianzhu
Date Issued
January 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Zheng, Dahai; Soh, Boon-Seng; Yin, Lu; Hu, Guangan; Chen, Qingfeng; Choi, Hyungwon; Han, Jongyoon; Chow, Vincent T. K. and Chen, Jianzhu. “Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro.” Scientific Reports 7 (January 2017): 41661 © 2017 The Author(s)
Version
Final published version
Abstract
Club cells are known to function as regional progenitor cells to repair the bronchiolar epithelium in response to lung damage. By lineage tracing in mice, we have shown recently that club cells also give rise to alveolar type 2 cells (AT2s) and alveolar type 1 cells (AT1s) during the repair of the damaged alveolar epithelium. Here, we show that when highly purified, anatomically and phenotypically confirmed club cells are seeded in 3-dimensional culture either in bulk or individually, they proliferate and differentiate into both AT2- and AT1-like cells and form alveolar-like structures. This differentiation was further confirmed by transcriptomic analysis of freshly isolated club cells and their cultured progeny. Freshly isolated club cells express Sca-1 and integrin α6, markers commonly used to characterize lung stem/progenitor cells. Together, current study for the first time isolated highly purified club cells for in vitro study and demonstrated club cells’ capacity to differentiate into alveolar epithelial cells at the single-cell level.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/srep41661
