Virtual microfluidics for digital quantification and single-cell sequencing
Name
Virtual microfluidics.pdf
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1.74 MB
Format
Adobe PDF
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Author(s)
Xu, Liyi
Brito, Ilana Lauren
Alm, Eric J
Blainey, Paul C
Date Issued
August 2016
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Xu, Liyi et al. “Virtual Microfluidics for Digital Quantification and Single-Cell Sequencing.” Nature Methods 13.9 (2016): 759–762.
Version
Author's final manuscript
Abstract
We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmeth.3955
