Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture
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Bettinger-2009-Nanofabricated colla.pdf
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Author(s)
Bettinger, Christopher J.
Kulig, Katherine M.
Vacanti, Joseph P.
Langer, Robert
Borenstein, Jeffrey T.
Date Issued
July 2009
Journal
Tissue Engineering. Part A
Publisher
Mary Ann Liebert, Inc.
Citation
Bettinger, Christopher J. et al. “Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture.” Tissue Engineering Part A 15.6 (2009): 1321-1329. ©2009 Mary Ann Liebert, Inc.
Version
Final published version
Abstract
Synthetic substrates that mimic the properties of extracellular matrix proteins hold significant promise for use in systems designed for tissue engineering applications. In this report, we designed a synthetic polymeric substrate that is intended to mimic chemical, mechanical, and topological characteristics of collagen. We found that elastomeric poly(ester amide) substrates modified with replica-molded nanotopographic features enhanced initial attachment, spreading, and adhesion of primary rat hepatocytes. Further, hepatocytes cultured on nanotopographic substrates also demonstrated reduced albumin secretion and urea synthesis, which is indicative of strongly adherent hepatocytes. These results suggest that these engineered substrates can function as synthetic collagen analogs for in vitro cell culture.
MIT Department
Charles Stark Draper Laboratory
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1089/ten.tea.2008.0134
